Affordable Access

deepdyve-link
Publisher Website

Guidelines for panel design, optimization, and performance of whole blood multi-color flow cytometry of platelet surface markers.

Authors
  • Busuttil-Crellin, Xavier1, 2
  • McCafferty, Conor1
  • Van Den Helm, Suelyn1
  • Yaw, Hui Ping1, 2
  • Monagle, Paul1, 2, 3
  • Linden, Matthew4
  • Ignjatovic, Vera1, 2
  • 1 Haematology Research Laboratory, Murdoch Children's Research Institute , Melbourne, Australia. , (Australia)
  • 2 Department of Paediatrics, The University of Melbourne , Melbourne, Australia. , (Australia)
  • 3 Department of Clinical Haematology, Royal Children's Hospital , Melbourne, Australia. , (Australia)
  • 4 School of Biosciences, The University of Western Australia , Perth, Australia. , (Australia)
Type
Published Article
Journal
Platelets
Publisher
Informa UK (Taylor & Francis)
Publication Date
Oct 02, 2020
Volume
31
Issue
7
Pages
845–852
Identifiers
DOI: 10.1080/09537104.2019.1709630
PMID: 31906818
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Flow cytometry is a valuable tool in determining the phenotype and function of platelets accurately. The emergence of platelet flow cytometry in recent years provides an attractive alternative to other platelet analytical techniques, with advantages such as requiring small volumes and being highly sensitive to minimal changes in receptor function and expression. Here we present a methodical approach encompassing the stages in the development and optimization of platelet flow cytometry panels based on our extensive experience in this area.

Report this publication

Statistics

Seen <100 times