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Growth in serum-free medium improves isolation of Chlamydia pneumoniae.

Authors
  • M Maass
  • A Essig
  • R Marre
  • W Henkel
Publication Date
Nov 01, 1993
Source
PMC
Keywords
License
Unknown

Abstract

Infectivity titers were determined for eight Chlamydia pneumoniae strains simultaneously grown in serum-free and serum-supplemented cell culture media. Use of serum-free medium resulted in a 10- to 50-fold increase in the susceptibility of HL cells to chlamydial infection. Comparative primary isolation of a wild-type strain also produced higher inclusion counts in a serum-free environment. Serum-free cultivation is recommended to increase the efficiency of C. pneumoniae isolation from clinical material and to permit elementary body purification without interference caused by serum components.

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