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Graphene oxide disrupts the protein-protein interaction between Neuroligin/NLG-1 and DLG-1 or MAGI-1 in nematode Caenorhabditis elegans.

Authors
  • Zhao, Yunli1
  • Chen, He2
  • Yang, Yunhan2
  • Wu, Qiuli2
  • Wang, Dayong3
  • 1 Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing 210009, China; Department of Preventive Medicine, Bengbu Medical College, Bengbu 233030, China. , (China)
  • 2 Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing 210009, China. , (China)
  • 3 Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing 210009, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
The Science of the total environment
Publication Date
Jan 15, 2020
Volume
700
Pages
134492–134492
Identifiers
DOI: 10.1016/j.scitotenv.2019.134492
PMID: 31627046
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Graphene oxide (GO) is a carbon-based engineered nanomaterial (ENM). Using Caenorhabditis elegans as an animal model, we investigated the effect of GO exposure on protein-protein interactions. In nematodes, NLG-1/Neuroligin, a postsynaptic protein, acted only in the neurons to regulate the GO toxicity. In the neurons, DLG-1, a PSD-95 protein, and MAGI-1, a S-SCAM protein, were identified as the downstream targets of NLG-1 in the regulation of GO toxicity. PKC-1, a serine/threonine protein kinase C, further acted downstream of neuronal DLG-1 and MAGI-1 to regulate the GO toxicity. Co-immunoprecipitation analysis demonstrated the protein-protein interaction between NLG-1 and DLG-1 or MAGI-1. After GO expression, this protein-protein interaction between NLG-1 and DLG-1 or MAGI-1 was significantly inhibited. Therefore, our data raised the evidence to suggest the potential of GO exposure in disrupting protein-protein interactions in organisms. Copyright © 2019 Elsevier B.V. All rights reserved.

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