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Granulomatous response to Coxiella burnetii, the agent of Q fever: the lessons from gene expression analysis.

Authors
  • Faugaret, Delphine
  • Ben Amara, Amira
  • Alingrin, Julie
  • Daumas, Aurélie
  • Delaby, Amélie
  • Lépolard, Catherine
  • Raoult, Didier
  • Textoris, Julien
  • Mège, Jean-Louis
Type
Published Article
Journal
Frontiers in Cellular and Infection Microbiology
Publisher
Frontiers Media SA
Publication Date
Jan 01, 2014
Volume
4
Pages
172–172
Identifiers
DOI: 10.3389/fcimb.2014.00172
PMID: 25566510
Source
Medline
Keywords
License
Unknown

Abstract

The formation of granulomas is associated with the resolution of Q fever, a zoonosis due to Coxiella burnetii; however the molecular mechanisms of granuloma formation remain poorly understood. We generated human granulomas with peripheral blood mononuclear cells (PBMCs) and beads coated with C. burnetii, using BCG extracts as controls. A microarray analysis showed dramatic changes in gene expression in granuloma cells of which more than 50% were commonly modulated genes in response to C. burnetii and BCG. They included M1-related genes and genes related to chemotaxis. The inhibition of the chemokines, CCL2 and CCL5, directly interfered with granuloma formation. C. burnetii granulomas also expressed a specific transcriptional profile that was essentially enriched in genes associated with type I interferon response. Our results showed that granuloma formation is associated with a core of transcriptional response based on inflammatory genes. The specific granulomatous response to C. burnetii is characterized by the activation of type 1 interferon pathway.

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