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Gold nanoparticles as localization markers for direct and live imaging of particle absorption through a Caco-2 cell monolayer using dark-field microscopy.

Authors
  • Kobori, Toshiro1
  • Watanabe, Jun
  • Nakao, Hidenobu
  • 1 National Food Research Institute, National Agriculture and Food Research Organization, Kan-nondai, Tsukuba, Japan. [email protected] , (Japan)
Type
Published Article
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Publication Date
Jan 01, 2012
Volume
28
Issue
1
Pages
61–61
Identifiers
PMID: 22232226
Source
Medline
License
Unknown

Abstract

Recently, efforts have been made to reduce the size of food particles containing functional ingredients, since reducing the size is expected to improve intestinal absorption. However, the absorption mechanisms have yet to be fully clarified. Therefore, a microscopy-based method for studying interactions between the particles and intestinal cells is required. We optimized the experimental conditions for observing gold nanoparticles (AuNPs) on the surface of an unfixed Caco-2 cell using dark-field microscopy (DFM). Tight junctions were clearly visible with AuNPs on the cells, producing intense scattered light under DFM. This suggests that AuNPs could be used as localization markers to visualize particle absorption through Caco-2 cells.

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