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Goat PRND expression pattern suggests its involvement in early sex differentiation.

Authors
  • Kocer, A
  • Gallozzi, M
  • Renault, L
  • Tilly, G
  • Pinheiro, I
  • Le Provost, F
  • Pailhoux, E
  • Vilotte, J L
Type
Published Article
Journal
Developmental dynamics : an official publication of the American Association of Anatomists
Publication Date
Mar 01, 2007
Volume
236
Issue
3
Pages
836–842
Identifiers
PMID: 17226816
Source
Medline
License
Unknown

Abstract

Expression of the goat prion protein gene locus was assessed by reverse transcriptase-polymerase chain reaction on testes and ovaries at various developmental stages. A weak and stochastic expression of the PRNP and PRNT genes was observed. For PRNT, it is consistent with the detected deletions of two single nucleotides within its open reading frame in ruminant genes. PRND was expressed in both tissues at all stages. Whereas its expression is constant in the ovaries, it increases in testes between 36 and 46 days postcoitum (dpc) and remains high thereafter. In testes, Doppel was found in the nucleus of germinal cells and in the cytoplasm of Leydig cells at 44 dpc. It was detected in the cytoplasm of Leydig cells and of some Sertoli and germinal cells at 62 dpc. In the ovaries, it was observed in the nucleus of germinal cells at 44 dpc and mainly in their cytoplasm at 62 dpc. This expression pattern was shown to parallel that of C-kit and suggests Doppel involvement in early testis differentiation.

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