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Glyphosate induces epithelial mesenchymal transition-related changes in human endometrial Ishikawa cells via estrogen receptor pathway.

Authors
  • Gastiazoro, M P1
  • Durando, M2
  • Milesi, M M2
  • Lorenz, V2
  • Vollmer, G3
  • Varayoud, J2
  • Zierau, O4
  • 1 Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral (UNL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Argentina; Institute for Zoology, Molecular Cell Physiology and Endocrinology, Technische Universität Dresden, Dresden, Germany. Electronic address: [email protected] , (Argentina)
  • 2 Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral (UNL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Argentina. , (Argentina)
  • 3 Institute for Zoology, Molecular Cell Physiology and Endocrinology, Technische Universität Dresden, Dresden, Germany. , (Germany)
  • 4 Institute for Zoology, Molecular Cell Physiology and Endocrinology, Technische Universität Dresden, Dresden, Germany. Electronic address: [email protected] , (Germany)
Type
Published Article
Journal
Molecular and cellular endocrinology
Publication Date
Apr 28, 2020
Volume
510
Pages
110841–110841
Identifiers
DOI: 10.1016/j.mce.2020.110841
PMID: 32360565
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Glyphosate based herbicides are the most commonly used herbicide in the world. We aimed to determine whether glyphosate (Gly) induces epithelial mesenchymal transition (EMT) - related changes in a human endometrial carcinoma cell line (Ishikawa cells), and whether the estrogen receptor (ER) pathway is involved in these changes. Ishikawa cells were exposed to Gly (0.2 μM and 2 μM) or 17β-estradiol (E2: 10-9 M). We detected that Gly increased cell migration and invasion ability compared to vehicle, as did E2. Moreover, a down regulation of E-cadherin mRNA expression was determined in response to Gly, similar to E2-effects. These results show that Gly promotes EMT-related changes in Ishikawa cells. When an ER antagonist (Fulvestrant: 10-7 M) was co-administrated with Gly, all changes were reversed, suggesting that Gly might promote EMT-related changes via ER-dependent pathway. Our results are interesting evidences of Gly effects on endometrial cancer progression via the ER-dependent pathway. Copyright © 2020 Elsevier B.V. All rights reserved.

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