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Glyphosate affects methylation in the promoter regions of selected tumor suppressors as well as expression of major cell cycle and apoptosis drivers in PBMCs (in vitro study).

Authors
  • Woźniak, Ewelina1
  • Reszka, Edyta2
  • Jabłońska, Ewa2
  • Balcerczyk, Aneta3
  • Broncel, Marlena4
  • Bukowska, Bożena5
  • 1 University of Lodz, Faculty of Biology and Environmental Protection, Department of Biophysics of Environmental Pollution, Pomorska Str. 141/143, 90-236 Lodz, Poland; Medical University of Lodz, Department of Internal Diseases and Clinical Pharmacology, Laboratory of Tissue Immunopharmacology, Kniaziewicza Str. 1/5, 91-347 Lodz, Poland. Electronic address: [email protected] , (Poland)
  • 2 Department of Molecular Genetics and Epigenetics, Nofer Institute of Occupational Medicine, Teresy Str. 8, 91-348 Lodz, Poland. , (Poland)
  • 3 University of Lodz, Faculty of Biology and Environmental Protection, Department of Molecular Biophysics, Pomorska Str. 141/143, 90-236 Lodz, Poland. , (Poland)
  • 4 Medical University of Lodz, Department of Internal Diseases and Clinical Pharmacology, Laboratory of Tissue Immunopharmacology, Kniaziewicza Str. 1/5, 91-347 Lodz, Poland. , (Poland)
  • 5 University of Lodz, Faculty of Biology and Environmental Protection, Department of Biophysics of Environmental Pollution, Pomorska Str. 141/143, 90-236 Lodz, Poland. , (Poland)
Type
Published Article
Journal
Toxicology in vitro : an international journal published in association with BIBRA
Publication Date
Nov 18, 2019
Volume
63
Pages
104736–104736
Identifiers
DOI: 10.1016/j.tiv.2019.104736
PMID: 31751608
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We have determined the effect of glyphosate on selected epigenetic parameters and major cell cycle drivers in human peripheral blood mononuclear cells (PBMCs). The cells were incubated with glyphosate at 0.5, 10 and 100 μM. The analysis included: global DNA methylation, methylation in the promoter regions of tumor suppressor genes (P16, P21, TP53) and proto-oncogenes (BCL2, CCND1) by the Real-Time PCR and the expression profile of the indicated genes by Real-Time PCR. The obtained results have revealed significant reduction of global DNA methylation level in PBMCs exposed to glyphosate. Tested compound changed methylation pattern of the P21 and TP53 suppressor gene promoters, but in case of other analyzed genes: P16, BCL2 and CCND1 we did not identify any statistically significant changes. Gene profiling showed that glyphosate changed the expression of genes involved in the regulation of cell cycle and apoptosis. Glyphosate decreased expression of P16 and TP53 as well as an increase in the expression of BCl2, CCND1 and P21. Summing up, our results have shown a potential disturbance in methylation processes and gene expression in human PBMCs exposed to glyphosate, but the observed changes do not prejudge about the final metabolic effects, which are depended on many other factors. Copyright © 2019 Elsevier Ltd. All rights reserved.

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