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The glycosyl moiety of lectin from sainfoin ( Onobrychis vichfolia, scop.)

Authors
  • Namen, Anthony E.
  • Hapner, Kenneth D.
Type
Published Article
Journal
BBA - Protein Structure
Publication Date
Jan 01, 1979
Volume
580
Issue
1
Pages
198–209
Identifiers
DOI: 10.1016/0005-2795(79)90210-1
Source
Elsevier
Keywords
License
Unknown

Abstract

A lectin isolated from the seeds of sainfoin ( Onobrychis viciifolia, Scop. var Eski) has been shown to be a glycoprotein containing 2.6% (w/w) neutral carbohydrate and 1.6% (w/w) glucosamine (Hapner, K.D. and Robbins, J.E. (1979) Biochim. Biophys. Acta 580, 186–197) A homogeneous glycopeptide accounting for 70% of the original glycoprotein carbohydrate was isolated from pronase digests of the lectin by gel filtration chromatography. Gas-liquid chromatographic and amino acid analyses showed the glycosyl portion to contain glucosamine, mannose, xylose and fucose in molar ratio to glycopeptide of 1.8 : 1.8 : 0.7 : 0.9. The amino acid sequence was determined as H 2N-Ser-Asn(glycosyl)-Glu-Thr-COOH. The glycosyl moiety was attached to the peptide through N-glycosidic linkage between asparagine and glucosamine.

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