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Glutamyl- and Glutaminyl-tRNA Synthetases Are a Promising Target for the Design of an L-Threonine–Producing Strain

Authors
  • Bubnov, D. M.1
  • Yuzbashev, T. V.1, 2
  • Fedorov, A. S.1
  • Bondarenko, F. V.1
  • Savchenko, A. S.3
  • Vybornaya, T. V.1
  • Filippova, S. S.1
  • Sineoky, S. P.1
  • 1 State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (Kurchatov Institute NRC, GOSNIIgenetika), Moscow, 117545, Russia , Moscow (Russia)
  • 2 Department of Bioengineering, Imperial College of London, London, SW72AZ, Great Britain , London (United Kingdom)
  • 3 Department of Integrated Biosciences, University of Tokyo, Tokyo, Japan , Tokyo (Japan)
Type
Published Article
Journal
Applied Biochemistry and Microbiology
Publisher
Pleiades Publishing
Publication Date
Dec 01, 2020
Volume
56
Issue
8
Pages
837–846
Identifiers
DOI: 10.1134/S0003683820080037
Source
Springer Nature
Keywords
License
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Abstract

AbstractThe present work describes an approach that uses a reduction in biomass accumulation during fermentation to improve the properties of a strain producing L-threonine. Glutamyl- and glutaminyl-tRNA synthetases were chosen as targets. Mutants carrying temperature-sensitive alleles of the mentioned enzymes were obtained. It was shown with this system that suppression of the function of tRNA synthetases led to the rapid arrest of culture growth and an increase in the productivity and conversion of L-threonine synthesis. One of the temperature-sensitive strains was used to obtain mutants with the ts phenotype under nonpermissive conditions. Some of these mutants accumulated less biomass and produced 10–12% more threonine than the original strain.

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