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GLUT2 and hexokinase control proximodistal gradient of intestinal glucose metabolism in the newborn pig

Authors
  • Cherbuy, C.
  • Darcy-Vrillon, B.
  • Posho, L.
  • Vaugelade, P.
  • Morel, M.T.
  • Bernard, Frances
  • Armelle Leturque
  • Pénicaud, Luc
  • Due, P.H.
Type
Published Article
Journal
American Journal of Physiology-Gastrointestinal and Liver Physiology
Publisher
American Physiological Society
Publication Date
Jun 22, 1997
Volume
272
Issue
6
Identifiers
DOI: 10.1152/ajpgi.1997.272.6.G1530
PMID: 9227491
OAI: oai:HAL:hal-00399362v1
Source
USPC - SET - SVS
Keywords
License
Green
External links

Abstract

We have reported previously that a high glycolytic capacity develops soon after birth in enterocytes isolated from suckling newborn pigs. In the present work, we investigated whether such metabolic changes could affect intestinal glucose utilization in vivo and examined possible variations in glucose metabolism along the small intestine. Glucose utilization by individual tissues was assessed using the 2-deoxyglucose technique. The overall glucose utilization rate was doubled in suckling vs. fasting 2-day-old pigs because of significantly higher rates in all tissues studied, except for the brain. In parallel, enterocytes were isolated from the proximal, medium, or distal jejunoileum of newborn vs. 2-day-old pigs and assessed for their capacity to utilize, transport, and phosphorylate glucose. Intestinal glucose consumption accounted for approximately 15% of glucose turnover rate in suckling vs. 8% in fasting pigs. Moreover, there was a proximal-to-distal gradient of glucose utilization in the intestinal mucosa of suckling pigs. Such a gradient was also evidenced on isolated enterocytes. The stimulation of both hexokinase activity (HK2 isoform) and basolateral glucose transporter (GLUT2), as observed in the proximal jejunum, could account for such a site-specific effect of suckling.

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