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Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae.

Authors
  • van Berkel, M A
  • Caro, L H
  • Montijn, R C
  • Klis, F M
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jul 25, 1994
Volume
349
Issue
1
Pages
135–138
Identifiers
PMID: 8045291
Source
Medline
License
Unknown

Abstract

Extension of a reporter protein with the carboxyterminal thirty amino acids of the cell wall mannoprotein alpha-agglutinin of Saccharomyces cerevisiae resulted in incorporation of the chimeric protein in the cell wall. By Western analysis it was shown that the incorporated protein contained beta-1,6-glucan similar to endogenous cell wall proteins, whereas excreted reporter protein was not glucosylated. This suggests that beta-1,6-glucan is involved in anchoring mannoproteins in the cell wall.

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