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Glial growth factor-2 promotes the survival, migration and bromodeoxyuridine incorporation of mammalian neural crest cells in caudal neural tube explant cultures.

Authors
  • Bannerman, P G
  • Puhalla, S
  • Sahai, A
  • Shieh, A
  • Berman, M
  • Pleasure, D
Type
Published Article
Journal
Developmental Brain Research
Publisher
Elsevier
Publication Date
Nov 30, 2000
Volume
124
Issue
1-2
Pages
93–99
Identifiers
PMID: 11113516
Source
Medline
License
Unknown

Abstract

Using an in vitro assay system, we found that GGF-2 increases the number of nascent trunk neural crest cells (NCC) present in the dorsal outgrowth derived from E12 caudal neural tube explants. Data is presented which suggests that this increased outgrowth was due to a combination of GGF-2 mediated effects, including its ability to promote (A) NCC survival by decreasing the percentage of NCC that undergo cell death via a mechanism involving DNA fragmentation, (B) the initial phases of NCC migration, (C) mitosis of peripherally migrating NCC. We also show that GGF-2 can promote the long-term survival of NCC in the absence of the neural tube. An immunohistochemical analysis indicates that NCC express erbB-2 and erbB-4 neuregulin receptors.

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