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Ginsenosides May Enhance the Functionality of Human Embryonic Stem Cell–Derived Cardiomyocytes In Vitro

Authors
  • Kim, Yoon Young1, 1
  • Ku, Jun Beom2
  • Liu, Hung Ching3
  • Ku, Seung-Yup1, 1
  • Kim, Seok Hyun1, 1
  • Choi, Young Min1, 1
  • 1 Seoul National University, Seoul, Korea , Seoul (South Korea)
  • 2 Choate Rosemary Hall, CT, New Haven, USA , New Haven (United States)
  • 3 Cornell University Weill Medical College, New York, NY, USA , New York (United States)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Oct 01, 2014
Volume
21
Issue
10
Pages
1312–1318
Identifiers
DOI: 10.1177/1933719114525269
Source
Springer Nature
Keywords
License
Yellow

Abstract

Various chemicals have been reported to induce the differentiation of human embryonic stem cells (hESCs) into cardiomyocytes (CMs), however, their contributions to the functionality of hESC-derived CMs are still limited. In this study, we evaluated the effects of red ginseng extract (RGE), ginsenoside-Rb1 (gRb1, panaxadiol), and ginsenoside-Re (gRe, panaxatriol) on the differentiation of hESCs and the functionality of derived CMs. Undifferentiated hESCs were treated with 0.25 mg/mL RGE, 10 μmol/L gRb1, or 10 μmol/L gRe for 48 hours at the differentiation induction (early stage) or maturation (late stage) period. The expression of mesodermal and cardiac transcription factor genes was upregulated in the ginsenoside-treated groups from early stage. The expression of cardiac sarcomeric genes was significantly upregulated at the late stage. The gRb1- and gRe-treated groups upregulated the expression of potassium voltage-gated channel subfamily E member 1 (KCNE1) and the gRe-treated group showed a longer beating duration compared to the control. Taken together, ginsenosides may enhance the functionality of hESC-derived CMs in vitro.

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