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Ginsenoside Rg3 protects heart against isoproterenol-induced myocardial infarction by activating AMPK mediated autophagy.

Authors
  • Sun, Gui-Zhi1
  • Meng, Fan-Ji1
  • Cai, Huai-Qiu2
  • Diao, Xue-Bo3
  • Zhang, Bo1
  • Bai, Xiu-Ping1
  • 1 Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. , (China)
  • 2 Department of Ultrasonography, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. , (China)
  • 3 Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. , (China)
Type
Published Article
Journal
Cardiovascular diagnosis and therapy
Publication Date
Apr 01, 2020
Volume
10
Issue
2
Pages
153–160
Identifiers
DOI: 10.21037/cdt.2020.01.02
PMID: 32420095
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Panax ginseng is a well-known medicinal herb that is widely used in traditional Chinese medicine for treating various diseases. Ginsenoside Rg3 (Rg3) is thought to be one of the most important active ingredients of Panax ginseng. However, the molecular mechanism underlying the beneficial effects of Rg3 has been elusive. In the mouse heart injury model induced by isoproterenol (ISO), we used brain natriuretic peptide (BNP), lactate dehydrogenase (LDH) and caspase-3 ELISA kits to test myocardium injury. To test whether Rg3 protects myocardial injury through AMPK mediated autophagy, we used specific AMPK inhibitor in combination with Rg3. NLRP3 inflammasome related molecules such as NLRP3, ASC and caspase-1 were measured by western-blot following Rg3 treatment. We found that Rg3 significantly reduced ISO induced myocardial injury indicated by the downregulation of serum BNP and LDH. In addition, we showed that the improvement of myocardial injury by Rg3 was associated with enhanced expression of autophagy related protein and activation of AMPK downstream signaling pathway. We observed that inhibition of AMPK significantly reversed the myocardial protective effect of Rg3, which is associated with a decrease of Rg3 induced autophagy. These together suggested that Rg3 may improve myocardial injury during MI through AMPK mediated autophagy. Our study also provides important translational evidence for using Rg3 in treating myocardial infarction (MI). 2020 Cardiovascular Diagnosis and Therapy. All rights reserved.

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