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GINS2 Functions as a Key Gene in Lung Adenocarcinoma by WGCNA Co-Expression Network Analysis.

Authors
  • Tian, Wen1, 2
  • Yang, Xianglin1, 2
  • Yang, He1, 2
  • Zhou, Baosen1, 2
  • 1 Department of Clinical Epidemiology, First Affiliated Hospital, China Medical University, Shenyang, People's Republic of China. , (China)
  • 2 Department of Epidemiology, School of Public Health, China Medical University, Shenyang, Liaoning 110001, People's Republic of China. , (China)
Type
Published Article
Journal
OncoTargets and Therapy
Publisher
Dove Medical Press
Publication Date
Jan 01, 2020
Volume
13
Pages
6735–6746
Identifiers
DOI: 10.2147/OTT.S255251
PMID: 32753902
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Lung adenocarcinoma is one of the malignant tumors in the world. This study aimed to explore the biological mechanism of GINS2 in lung adenocarcinoma. Raw data were downloaded from GEO. WGCNA co-expression network and PPI network were established to identify the hub gene. The expression profile and clinical features of GINS2 were collected from TCGA-LUAD cohort. Survival analysis in TCGA-LUAD cohort was plotted by R package. GSEA was analyzed via GSEA software. MTS, Transwell and apoptosis assays were used to detect the proliferation, migration and apoptotic abilities of lung adenocarcinoma cells. GINS2 was identified as the hub gene via WGCNA co-expression network and PPI network. Higher GINS2 expressions were observed in TCGA-LUAD cohort, GSE32863 and clinical samples dataset. Overexpression of GINS2 had a significantly negative connection with poor survival outcome. GSEA results revealed that GINS2 could be enriched in "HALLMARK_G2M_CHECKPOINT", "HALLMARK_E2F_TARGETS", "HALLMARK_DNA_REPAIR" and "HALLMARK_MYC_TARGETS_V2". Overexpression of GINS2 promoted tumor cell proliferation and migration and suppressed cell apoptosis. Our results explored that GINS2 functioned as an oncogene in lung adenocarcinoma, and suggested that GINS2 could act as a promising prognosis biomarker for lung adenocarcinoma. © 2020 Tian et al.

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