Affordable Access

Access to the full text

Genotyping and characterization of prophage patterns in clinical isolates of Staphylococcus aureus

Authors
  • Dini, Mahya1
  • Shokoohizadeh, Leili1
  • Jalilian, Farid Aziz2
  • Moradi, Abbas3
  • Arabestani, Mohammad Reza1, 4
  • 1 University of Hamadan, Department of Microbiology, Faculty of Medicine, School of Medicine, Hamadan, Iran , Hamadan (Iran)
  • 2 University of Hamadan, Department of Virology, School of Medicine, Hamadan, Iran , Hamadan (Iran)
  • 3 Hamadan University of Medical Sciences, Department of Community Medicine, Hamadan, Iran , Hamadan (Iran)
  • 4 Hamadan University of Medical Sciences, Nutritious Research Center, Hamadan, Iran , Hamadan (Iran)
Type
Published Article
Journal
BMC Research Notes
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Oct 21, 2019
Volume
12
Issue
1
Identifiers
DOI: 10.1186/s13104-019-4711-4
Source
Springer Nature
Keywords
License
Green

Abstract

ObjectiveStaphylococcus aureus is considered an important pathogen with a variety of virulence factors in communities and hospitals all around the world. Prophage typing is a practical technique for categorizing this bacterium. In this study, we focused on the detection of prophage patterns in methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) strains based on their virulence factors, antimicrobial resistance patterns, and molecular typing by rep-PCR.ResultsOut of 126 S. aureus isolates, 45 (35.7%) were identified as MRSA. In total, 17 different prophage types were detected and 112 strains out of 126 strains contained at least one prophage. There was a statistically significant relationship between hld, hlg, eta and SGA, SGA, and SGFb, respectively. The results of the rep-PCR analysis revealed 14 different patterns among the MRSA and MSSA isolates. In conclusion, the presence of different prophage-encoded virulence factors and antibiotic-resistant genes among MRSA strains enables them to produce a broad range of diseases. Thus, diverse MRSA strains which have these prophages can be considered as a potential threat to the patient’s health in either the hospital or the community.

Report this publication

Statistics

Seen <100 times