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Genotypic and Resistance Profile Analysis of Two Oat Crown Rust Differential Sets Urge Coordination and Standardization.

Authors
  • Nguyen, Duong T1, 2
  • Henningsen, Eva C1, 3
  • Lewis, David1
  • Mago, Rohit1
  • McNeil, Meredith4
  • Suchecki, Radoslaw2
  • Boden, Scott5
  • Sperschneider, Jana1
  • Kianian, Shahryar F6
  • Dodds, Peter N1
  • Figueroa, Melania1
  • 1 Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, ACT 2601, Australia. , (Australia)
  • 2 Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Adelaide, SA 5064, Australia. , (Australia)
  • 3 Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia. , (Australia)
  • 4 Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, St. Lucia, QLD 4067, Australia. , (Australia)
  • 5 School of Agriculture, Food & Wine, Faculty of Sciences, Waite Research Institute, University of Adelaide, Adelaide, SA 5064, Australia. , (Australia)
  • 6 U.S. Department of Agriculture-Agricultural Research Service, Cereal Disease Laboratory, St. Paul, MN, U.S.A.
Type
Published Article
Journal
Phytopathology
Publisher
Scientific Societies
Publication Date
Jun 01, 2024
Volume
114
Issue
6
Pages
1356–1365
Identifiers
DOI: 10.1094/PHYTO-10-23-0353-R
PMID: 38114076
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Puccinia coronata f. sp. avenae is the causal agent of the disease known as crown rust, which represents a bottleneck in oat production worldwide. Characterization of pathogen populations often involves race (pathotype) assignments using differential sets, which are not uniform across countries. This study compared the virulence profiles of 25 P. coronata f. sp. avenae isolates from Australia using two host differential sets, one from Australia and one from the United States. These differential sets were also genotyped using diversity arrays technology sequencing technology. Phenotypic and genotypic discrepancies were detected on 8 out of 29 common lines between the two sets, indicating that pathogen race assignments based on those lines are not comparable. To further investigate molecular markers that could assist in the stacking of rust resistance genes important for Australia, four published Pc91-linked markers were validated across the differential sets and then screened across a collection of 150 oat cultivars. Drover, Aladdin, and Volta were identified as putative carriers of the Pc91 locus. This is the first report to confirm that the cultivar Volta carries Pc91 and demonstrates the value of implementing molecular markers to characterize materials in breeding pools of oat. Overall, our findings highlight the necessity of examining seed stocks using pedigree and molecular markers to ensure seed uniformity and bring robustness to surveillance methodologies. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

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