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Genotoxicity evaluation of tobacco and nicotine delivery products: Part Two. In vitro micronucleus assay.

Authors
  • Thorne, David1
  • Leverette, Robert2
  • Breheny, Damien3
  • Lloyd, Mel4
  • McEnaney, Stephen4
  • Whitwell, James4
  • Clements, Julie4
  • Bombick, Betsy2
  • Gaça, Marianna3
  • 1 British American Tobacco, R&D, Southampton, Hampshire, SO15 8TL, UK. Electronic address: [email protected]
  • 2 RAI Services Company, 401 N. Main Street, Winston-Salem, NC, 27101, USA.
  • 3 British American Tobacco, R&D, Southampton, Hampshire, SO15 8TL, UK.
  • 4 Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire, HG3 1PY, UK.
Type
Published Article
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
Publication Date
Oct 01, 2019
Volume
132
Pages
110546–110546
Identifiers
DOI: 10.1016/j.fct.2019.05.054
PMID: 31163219
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this study, a variety of test matrices from tobacco and nicotine delivery products were assessed against a 3R4F Kentucky reference cigarette using the in vitro micronucleus assay. Testing was conducted using two Chinese hamster cell lines (CHO and V79), and a human lymphoblastoid cell line (TK6), in accordance with established guidelines. Total particulate matter (TPM) from a 3R4F Reference cigarette was compared to an electronic cigarette e-liquid, electronic cigarette TPM and TPM from a commercial tobacco heating product using a standard and an extended treatment condition with recovery period. Cells were assessed with 3R4F TPM prior to assessment of the other tobacco and nicotine product test matrices. These cell lines gave varied responses to 3R4F TPM with the most robust response using V79 cells. The use of an extended exposure/recovery period was seen to increase assay sensitivity for CHO and V79 cell lines but was less clear for TK6 cells. Negative responses were observed for all products except 3R4F across all treatment conditions in V79 cells. The most potent response to cigarette smoke was following extended treatment with recovery, suggesting this may be a more appropriate treatment for the future assessment of tobacco and nicotine product test matrices. Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.

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