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Genomic Rewiring of SOX2 Chromatin Interaction Network during Differentiation of ESCs to Postmitotic Neurons

Authors
  • Bunina, Daria1, 2
  • Abazova, Nade2, 3, 4
  • Diaz, Nichole2
  • Noh, Kyung-Min2
  • Krijgsveld, Jeroen3, 5
  • Zaugg, Judith B.1
  • 1 Structural and Computational Biology Unit, European Molecular Biology Laboratory, EMBL, Meyerhofstrasse 1 Heidelberg 69117, Germany
  • 2 Genome Biology Unit, European Molecular Biology Laboratory, EMBL, Meyerhofstrasse 1 Heidelberg 69117, Germany
  • 3 Proteomics of Stem Cells and Cancer, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany
  • 4 Collaboration for joint PhD degree between the European Molecular Biology Laboratory and Heidelberg University, Faculty of Biosciences, Heidelberg, Germany
  • 5 Heidelberg University, Medical Faculty Heidelberg University, Faculty of Biosciences, Heidelberg, Germany
Type
Published Article
Journal
Cell Systems
Publisher
Cell Press
Publication Date
Jun 24, 2020
Volume
10
Issue
6
Pages
480–494
Identifiers
DOI: 10.1016/j.cels.2020.05.003
PMID: 32553182
PMCID: PMC7322528
Source
PubMed Central
Keywords
License
Unknown

Abstract

In this work, Bunina et al. profiled transcriptome, proteome, and chromatin accessibility dynamics during differentiation of mouse embryonic stem cells to postmitotic neurons. They revealed extensive associations between molecular layers within and across differentiation time points and uncovered an unexpected interaction of two chromatin-bound proteins, SOX2 and ATRX, in neurons. Their role in regulating neuronal genes was validated by CRISPR. The study highlights the significance of multi-omic approaches followed by specific functional experiments in understanding gene regulation in complex systems.

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