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Genomic comparison of Clostridium chauvoei isolates from classical and visceral clinical manifestation.

Authors
  • Ziech, Rosangela Estel1
  • Siqueira, Franciele Maboni2
  • Cibulski, Samuel3
  • De Carli, Silvia4
  • Dos Santos, Helton Fernandes5
  • Fighera, Rafael Almeida5
  • Frey, Joachim6
  • de Vargas, Agueda Castagna7
  • 1 Laboratório de Bacteriologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. [email protected] , (Brazil)
  • 2 Laboratório de Bacteriologia Veterinária, Departamento de Patologia Clínica Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. , (Brazil)
  • 3 Laboratório de Virologia, UFRGS, Porto Alegre, RS, Brazil. , (Brazil)
  • 4 Laboratório de Diagnóstico Molecular, Universidade Luterana do Brasil, Canoas, RS, Brazil. , (Brazil)
  • 5 Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. , (Brazil)
  • 6 Vetsuisse Faculty, University of Bern, Bern, Switzerland. , (Switzerland)
  • 7 Laboratório de Bacteriologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. , (Brazil)
Type
Published Article
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Date
Sep 01, 2020
Volume
51
Issue
3
Pages
1327–1332
Identifiers
DOI: 10.1007/s42770-019-00177-0
PMID: 31650466
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Clostridium chauvoei is the etiological agent of blackleg, an infectious disease affecting cattle and small ruminants worldwide. This disease can manifest as classical blackleg, a condition in which skeletal muscles are affected and visceral blackleg, which affects the heart, sublingual muscles, and the diaphragm. The pathogenesis of the visceral form of the disease is poorly understood. The objective of this study is to determine and analyze complete genomic sequences of six C. chauvoei strains, five isolates from skeletal muscle and one isolate from a visceral case of blackleg in Brazil, to provide insights into the differences in pathogenic profiles of strains causing the different forms of disease. The full genomes of the six C. chauvoei strains were sequenced and comparative analyses were performed among these genomes and the C. chauvoei reference strain JF4335. The results of this study revealed that the genomes of the C. chauvoei strains analyzed are highly conserved; no particular differences were noted that could be associated with the two different clinical manifestations of the disease.

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