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Genomic analysis of a Trichoplusia ni Betabaculovirus (TnGV) with three different viral enhancing factors and two unique genes

Authors
  • de los Ángeles Bivian-Hernández, Ma.1
  • López-Tlacomulco, Juventino2
  • Mares-Mares, Everardo1
  • Ibarra, Jorge E.2
  • Del Rincón-Castro, María Cristina1
  • 1 Universidad de Guanajuato, Posgrado en Biociencias, División de Ciencias de la Vida, Departamento de Alimentos, Campus Irapuato-Salamanca, Ex Hacienda El Copal Km. 9.0, Carretera Irapuato-León, Irapuato, Guanajuato, Mexico , Irapuato (Mexico)
  • 2 CINVESTAV-Irapuato, Apartado Postal 629, Irapuato, Guanajuato, 36500, Mexico , Irapuato (Mexico)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
Aug 30, 2017
Volume
162
Issue
12
Pages
3705–3715
Identifiers
DOI: 10.1007/s00705-017-3506-y
Source
Springer Nature
License
Yellow

Abstract

The complete genome of a Trichoplusia ni granulovirus (TnGV) is described and analyzed. The genome contains 175,360 bp (KU752557), becoming the third largest genome within the genus Betabaculovirus, smaller only than the Xestia c-nigrum GV (XecnGV) (178,733 pb) and the Pseudaletia unipuncta GV (PsunGV) (176,677 pb) genomes. The TnGV genome has a 39.81% C+G content and a total of 180 ORFs were identified, 96 of them in the granulin gene direction and 84 in the opposite direction. A total of 94.38% of the ORFs showed high identity with those of ClanGV, HaGV, and SlGV. Eight homologous regions (hrs) were identified as well as one apoptosis inhibitor (IAP-3). Interestingly, three viral enhancing factors (VEFs) were located in TnGV genome: VEF-1 (orf153), VEF-3 (orf155), and VEF-4 (orf164), additional to another metalloprotease (orf37). Two ORFs were unique to TnGV (orf100 and orf101) and another one was shared by only TnGV and AgseGV (orf2). Eleven of the deduced proteins showed high identity with proteins from nucleopolyhedroviruses, three with proteins from ascoviruses, and one with an entomopoxvirus protein. The largest deduced protein contains 1,213 amino acids (orf43) and the smallest deduced protein contains only 50 amino acids (orf143). Sequence identity and phylogenetic analyses showed that the closest related genomes to TnGV are, to date, those of PsunGV and XecnGV. This genome analysis may contribute to functional research on TnGV, and may form the bases for the utilization of this betabaculovirus as a pest control agent.

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