Affordable Access

deepdyve-link
Publisher Website

A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth.

Authors
  • Jiang, Yale1, 2, 3
  • Forno, Erick1, 2
  • Han, Yueh-Ying1, 2
  • Xu, Zhongli1, 2, 3
  • Hu, Donglei4
  • Boutaoui, Nadia1, 2
  • Eng, Celeste4
  • Acosta-Pérez, Edna5
  • Huntsman, Scott4
  • Colón-Semidey, Angel6
  • Keys, Kevin L4, 7
  • Rodríguez-Santana, José R8
  • Alvarez, María6
  • Pino-Yanes, Maria9, 10, 11
  • Canino, Glorisa5
  • Chen, Wei1, 2
  • Burchard, Esteban G4, 12
  • Celedón, Juan C1, 2
  • 1 Division of Pulmonary Medicine, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA, USA.
  • 2 Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • 3 School of Medicine, Tsinghua University, Beijing, China. , (China)
  • 4 Department of Medicine, University of California San Francisco, San Francisco, CA, USA.
  • 5 Behavioral Sciences Research Institute, Medical Science Campus, University of Puerto Rico, San Juan, PR. , (Puerto Rico)
  • 6 Department of Pediatrics, Medical Science Campus, University of Puerto Rico, San Juan, PR. , (Puerto Rico)
  • 7 Berkeley Institute for Data Science, University of California Berkeley, Berkeley, CA, USA.
  • 8 Centro de Neumología Pediátrica, San Juan, PR.
  • 9 Genomics and Health Group, Department of Biochemistry, Microbiology, Cell Biology and Genetics, Universidad de La Laguna, La Laguna, Santa Cruz de Tenerife, Spain. , (Spain)
  • 10 CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain. , (Spain)
  • 11 Instituto de Tecnologías Biomédicas (ITB), Universidad de La Laguna, San Cristóbal de La Laguna, Santa Cruz de Tenerife, Spain. , (Spain)
  • 12 Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Type
Published Article
Journal
Epigenetics
Publisher
Landes Bioscience
Publication Date
May 01, 2021
Volume
16
Issue
5
Pages
577–585
Identifiers
DOI: 10.1080/15592294.2020.1809872
PMID: 32799603
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Latinos are heavily affected with childhood asthma. Little is known about epigenetic mechanisms of asthma in Latino youth. We conducted a meta-analysis of two epigenome-wide association studies (EWAS) of asthma, using DNA from white blood cells (WBCs) from 1,136 Latino children and youth aged 6 to 20 years. Genes near the top CpG sites in this EWAS were examined in a pathway enrichment analysis, and we then assessed whether our results replicated those from publicly available data from three independent EWAS conducted in non-Latino populations. We found that DNA methylation profiles differed between subjects with and without asthma. After adjustment for covariates and multiple testing, two CpGs were differentially methylated at a false discovery rate (FDR)-adjusted P < 0.1, and 193 CpG sites were differentially methylated at FDR-adjusted P < 0.2. The two top CpGs are near genes relevant to inflammatory signalling, including CAMK1D (Calcium/Calmodulin Dependent Protein Kinase ID) and TIGIT (T Cell Immunoreceptor With Ig And ITIM Domains). Moreover, 25 genomic regions were differentially methylated between subjects with and without asthma, at Šidák-corrected P < 0.10. An enrichment analysis then identified the TGF-beta pathway as most relevant to asthma in our analysis, and we replicated some of the top signals from publicly available EWAS datasets in non-Hispanic populations. In conclusion, we have identified novel epigenetic markers of asthma in WBCs from Latino children and youth, while also replicating previous results from studies conducted in non-Latinos.

Report this publication

Statistics

Seen <100 times