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A genome-wide association study on growth traits in orange-spotted grouper (Epinephelus coioides) with RAD-seq genotyping

Authors
  • Yu, Hui1, 2
  • You, Xinxin2
  • Li, Jia2
  • Zhang, Xinhui2
  • Zhang, Shuai2
  • Jiang, Shoujia2
  • Lin, Xueqiang2
  • Lin, Hao-Ran3
  • Meng, Zining3
  • Shi, Qiong1, 2, 4
  • 1 University of Chinese Academy of Sciences, BGI Education Center, Shenzhen, 518083, China , Shenzhen (China)
  • 2 BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen, 518083, China , Shenzhen (China)
  • 3 Sun Yat-Sen University, State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangzhou, 510275, China , Guangzhou (China)
  • 4 Shenzhen University, Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen, 518060, China , Shenzhen (China)
Type
Published Article
Journal
Science China Life Sciences
Publisher
Science China Press
Publication Date
Mar 12, 2018
Volume
61
Issue
8
Pages
934–946
Identifiers
DOI: 10.1007/s11427-017-9161-4
Source
Springer Nature
Keywords
License
Yellow

Abstract

The orange-spotted grouper, Epinephelus coioides, is one of the most popular fish in China and Southeast Asian countries because of its important economic value. However, molecular mechanism underlying the growth of orange-spotted grouper has never been fully understood. Herein, we performed a genome-wide association study (GWAS) on a natural population of 198 individuals aiming to screen the whole genome of orange-spotted grouper for identification of growth-related loci by restrictionsite associated DNA sequencing. In this research, 261,366 single nucleotide polymorphisms (SNPs) were developed, in which 110 SNPs were identified to be correlated with growth and 20 SNPs were further confirmed to be associated with both body weight and total length. From these identified SNPs, we annotated a total of 34 genes, including adgrb2, csnkza1, cers5, col22a1, creb5, dnd1, dzank1, dnai1, npy2r, fat3, lrrk2, lrp5, map3k9, and so on. Among these candidate genes, npy2r (neuropeptide Y receptor Y2) was reported to play a critical role in growth of the orange-spotted grouper. In addition, population structure, principal component analysis, kinship matrix and linkage disequilibrium were examined to verify the accuracy and reliability of our GWAS results. Our data will also provide a valuable genetic resource for further marker-assisted selection program to improve growth quality in groupers.

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