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Genome-wide analysis of Acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

Authors
  • Dassa, Bareket1
  • Borovok, Ilya2
  • Lamed, Raphael2
  • Henrissat, Bernard3
  • Coutinho, Pedro3
  • Hemme, Christopher L4
  • Huang, Yue4
  • Zhou, Jizhong4
  • Bayer, Edward A1
  • 1 The Weizmann Institute of Science, Department of Biological Chemistry, Rehovot, Israel , Rehovot (Israel)
  • 2 Tel Aviv University, Department of Molecular Microbiology and Biotechnology, Ramat Aviv, Tel Aviv, Israel , Ramat Aviv (Israel)
  • 3 CNRS and Universite Aix- Marseilles I & II, Architecture et Fonction des Macromolecules Biologiques, Marseilles, France , Marseilles (France)
  • 4 University of Oklahoma, Department of Botany and Microbiology, and Institute for Environmental Genomics, Norman, OK, USA , Norman (United States)
Type
Published Article
Journal
BMC Genomics
Publisher
Springer (Biomed Central Ltd.)
Publication Date
May 30, 2012
Volume
13
Issue
1
Identifiers
DOI: 10.1186/1471-2164-13-210
Source
Springer Nature
Keywords
License
Yellow

Abstract

BackgroundMicrobial degradation of plant cell walls and its conversion to sugars and other byproducts is a key step in the carbon cycle on Earth. In order to process heterogeneous plant-derived biomass, specialized anaerobic bacteria use an elaborate multi-enzyme cellulosome complex to synergistically deconstruct cellulosic substrates. The cellulosome was first discovered in the cellulolytic thermophile, Clostridium thermocellum, and much of our knowledge of this intriguing type of protein composite is based on the cellulosome of this environmentally and biotechnologically important bacterium. The recently sequenced genome of the cellulolytic mesophile, Acetivibrio cellulolyticus, allows detailed comparison of the cellulosomes of these two select cellulosome-producing bacteria.ResultsComprehensive analysis of the A. cellulolyticus draft genome sequence revealed a very sophisticated cellulosome system. Compared to C. thermocellum, the cellulosomal architecture of A. cellulolyticus is much more extensive, whereby the genome encodes for twice the number of cohesin- and dockerin-containing proteins. The A. cellulolyticus genome has thus evolved an inflated number of 143 dockerin-containing genes, coding for multimodular proteins with distinctive catalytic and carbohydrate-binding modules that play critical roles in biomass degradation. Additionally, 41 putative cohesin modules distributed in 16 different scaffoldin proteins were identified in the genome, representing a broader diversity and modularity than those of Clostridium thermocellum. Although many of the A. cellulolyticus scaffoldins appear in unconventional modular combinations, elements of the basic structural scaffoldins are maintained in both species. In addition, both species exhibit similarly elaborate cell-anchoring and cellulosome-related gene- regulatory elements.ConclusionsThis work portrays a particularly intricate, cell-surface cellulosome system in A. cellulolyticus and provides a blueprint for examining the specific roles of the various cellulosomal components in the degradation of complex carbohydrate substrates of the plant cell wall by the bacterium.

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