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Genome-wide examination of the natural solar radiation response in Shewanella oneidensis MR-1.

Authors
  • Qiu, Xiaoyun
  • Tiedje, James M
  • Sundin, George W
Type
Published Article
Journal
Photochemistry and Photobiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jan 01, 2005
Volume
81
Issue
6
Pages
1559–1568
Identifiers
PMID: 16022560
Source
Medline
License
Unknown

Abstract

We previously reported that Shewanella oneidensis MR-1 is extremely sensitive to natural solar radiation (NSR). Here we analyzed the global transcriptional profile of MR-1 during a 1-h recovering period after exposure to ambient solar light at a dose that yields about 20% survival rate on a Luria-Bertani (LB) plate. We observed the induction of DNA damage-repair genes, the SOS response as well as detoxification strategies that we previously observed in MR-1 following artificial UV-A irradiation. Few prophage-related genes were induced by natural solar UV radiation, however, in contrast to what was observed following artificial UV-B irradiation. Overall, the cellular response to NSR in MR-1 was more similar to that of UV-A than that of UV-B, but additional genes involved in detoxification were induced compared with induction by either UV-B or UV-A or their sum. Thus, oxidative stress appeared to contribute greatly to the NSR-induced cytotoxic effects in MR-1. A total of 29.1% of genome showed differential expression following NSR exposure, which is much greater than following exposure by UV-B (4.0%), UV-A (8.2%) or their sum (10.7%). Our data suggest that NSR may impact biological processes in a much more complex manner than previously thought.

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