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Genome sequence analysis of 91 Salmonella Enteritidis isolates from mice caught on poultry farms in the mid 1990s.

Authors
  • Guard, Jean1
  • Cao, Guojie2
  • Luo, Yan2
  • Baugher, Joseph D2
  • Davison, Sherrill3
  • Yao, Kuan2
  • Hoffmann, Maria2
  • Zhang, Guodong2
  • Likens, Nathan4
  • Bell, Rebecca L2
  • Zheng, Jie2
  • Brown, Eric2
  • Allard, Marc2
  • 1 US Department of Agriculture, US National Poultry Research Center, Athens, GA 30605,. Electronic address: [email protected]
  • 2 Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, MD 20740.
  • 3 School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104.
  • 4 US Department of Agriculture, US National Poultry Research Center, Athens, GA 30605.
Type
Published Article
Journal
Genomics
Publisher
Elsevier
Publication Date
Jan 01, 2020
Volume
112
Issue
1
Pages
528–544
Identifiers
DOI: 10.1016/j.ygeno.2019.04.005
PMID: 30974149
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A total of 91 draft genome sequences were used to analyze isolates of Salmonella enterica serovar Enteritidis obtained from feral mice caught on poultry farms in Pennsylvania. One objective was to find mutations disrupting open reading frames (ORFs) and another was to determine if ORF-disruptive mutations were present in isolates obtained from other sources. A total of 83 mice were obtained between 1995-1998. Isolates separated into two genomic clades and 12 subgroups due to 742 mutations. Nineteen ORF-disruptive mutations were found, and in addition, bigA had exceptional heterogeneity requiring additional evaluation. The TRAMS algorithm detected only 6 ORF disruptions. The sefD mutation was the most frequently encountered mutation and it was prevalent in human, poultry, environmental and mouse isolates. These results confirm previous assessments of the mouse as a rich source of Salmonella enterica serovar Enteritidis that varies in genotype and phenotype. Published by Elsevier Inc.

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