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Genistein crosses the bioartificial oviduct and alters secretion composition.

Authors
  • Simintiras, Constantine A1
  • Sturmey, Roger G2
  • 1 Hull York Medical School (HYMS), University of Hull, Cottingham Road, Hull, HU6 7RX, UK. Electronic address: [email protected]
  • 2 Hull York Medical School (HYMS), University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Type
Published Article
Journal
Reproductive toxicology (Elmsford, N.Y.)
Publication Date
Aug 01, 2017
Volume
71
Pages
63–70
Identifiers
DOI: 10.1016/j.reprotox.2017.04.010
PMID: 28461242
Source
Medline
Keywords
License
Unknown

Abstract

The dietary derived isoflavone and oestrogen analogue, genistein, is known to perturb fundamental reproductive events such as implantation and embryo cleavage. However the question of whether genistein is able to traverse the oviduct epithelial monolayer and impact oviduct fluid secretion remains unclear. This study tests these research questions using a bioartificial oviduct to show that genistein permeates the oviduct lumen in vitro with a biphasic (burst and plateau) kinetic profile, faster than spontaneous diffusion, and alters the amino acid composition of in vitro derived oviduct fluid (ivDOF) but not as an oestrogen analogue. In addition to offering insights into the potential mechanisms of these findings, this manuscript demonstrates the potential to use the bioartificial oviduct model to characterise the transport or barrier properties of the oviduct towards a range of circulating xenobiotics.

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