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Genetic variation in Fusarium merismoides preserved by two different methods.

Authors
  • Gaylarde, C
  • Kelley, J
Type
Published Article
Journal
World journal of microbiology & biotechnology
Publication Date
May 01, 1995
Volume
11
Issue
3
Pages
319–321
Identifiers
DOI: 10.1007/BF00367108
PMID: 24414657
Source
Medline
License
Unknown

Abstract

Restriction-enzyme digestion followed by agarose gel electrophoresis demonstrated differences between the purified DNA from Fusarium merismoides which had been held in a culture collection in liquid N2 and that from soil-stored samples of the same fungus. Extended incubation (3 to 4 weeks) of a culture resulted in the appearance of an extra band, sensitive to EcoRI, which could correspond to a previously reported plasmid and may, therefore, be associated with senescence. The results emphasize the importance of using cultures of a standard age for genetic analysis and demonstrate the potential of microbial preservation and storage techniques to affect the fungal genome.

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