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Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells.

Authors
  • Liu, Wei
  • Hou, Steven X
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2008
Volume
450
Pages
61–70
Identifiers
DOI: 10.1007/978-1-60327-214-8_4
PMID: 18370051
Source
Medline
License
Unknown

Abstract

The advancement of Drosophila germline stem cell research accompanies the development of powerful new tools for genetic analysis. These include the techniques of stem cell labeling, cell lineage tracing, mosaic mutant analysis, and gene manipulation in targeted cell populations, which together constitute the critical methodologies in stem cell research. We discuss four such techniques: the tubulin-lacZ positive-labeling system; the positively marked mosaic lineage (PMML) method; the flipase/flipase recombination target (FLP/FRT)-based mosaic mutant analysis; and the GAL80-based mosaic analysis with a repressible cell marker (MARCM) system.

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