Affordable Access

Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers.

Authors
  • Martins, M
  • Sarmento, D
  • Oliveira, M M
Type
Published Article
Journal
Plant cell reports
Publication Date
Dec 01, 2004
Volume
23
Issue
7
Pages
492–496
Identifiers
PMID: 15372197
Source
Medline
License
Unknown

Abstract

Almond shoots produced by axillary branching from clone VII derived from a seedling of cultivar Boa Casta were evaluated for somaclonal variation using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) analysis. To verify genetic stability we compared RAPD and ISSR patterns of plantlets obtained after 4 and 6 years of in vitro multiplication. A total of 64 RAPD and 10 ISSR primers gave 326 distinct and reproducible band classes, monomorphic across all 22 plantlets analysed. Thus, a total of 7,172 bands were generated, exhibiting homogeneous RAPD and ISSR patterns for the plantlets tested. These results suggest that the culture conditions used for axillary branching proliferation are appropriate for clonal propagation of almond clone VII, as they do not seem to interfere with the integrity of the regenerated plantlets. These results allowed us to establish the use of axillary branching plantlets (mother-plants) as internal controls for the analysis of somaclonal variation of shoots regenerated from other in vitro culture processes performed with clone VII (adventitious regeneration, regeneration from meristem culture, virus sanitation programs and genetic engineering).

Report this publication

Statistics

Seen <100 times