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Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates

Authors
  • Lê, Hương Giang1, 2
  • Thái, Thị Lam1, 2
  • Kang, Jung-Mi1, 2
  • Lee, Jinyoung3
  • Moe, Mya4
  • Võ, Tuấn Cường1, 2
  • Naw, Haung1, 2
  • Myint, Moe Kyaw4
  • Htun, Zaw Than4
  • Kim, Tong-Soo3
  • Shin, Ho-Joon5
  • Na, Byoung-Kuk1, 2
  • 1 Gyeongsang National University College of Medicine, Jinju, 52727, Republic of Korea , Jinju (South Korea)
  • 2 Gyeongsang National University, Jinju, 52727, Republic of Korea , Jinju (South Korea)
  • 3 Inha University College of Medicine, Incheon, 22212, Republic of Korea , Incheon (South Korea)
  • 4 Department of Medical Research Pyin Oo Lwin Branch, Pyin Oo Lwin, Myanmar , Pyin Oo Lwin (Myanmar (Burma))
  • 5 Ajou University School of Medicine, Suwon, 16499, Republic of Korea , Suwon (South Korea)
Type
Published Article
Journal
Malaria Journal
Publisher
Springer (Biomed Central Ltd.)
Publication Date
May 19, 2020
Volume
19
Issue
1
Identifiers
DOI: 10.1186/s12936-020-03256-y
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundPlasmodium falciparum merozoite surface protein-3 (PfMSP-3) is a target of naturally acquired immunity against P. falciparum infection and is a promising vaccine candidate because of its critical role in the erythrocyte invasion of the parasite. Understanding the genetic diversity of pfmsp-3 is important for recognizing genetic nature and evolutionary aspect of the gene in the natural P. falciparum population and for designing an effective vaccine based on the antigen.MethodsBlood samples collected from P. falciparum-infected patients in Naung Cho and Pyin Oo Lwin, Myanmar, in 2015 were used in this study. The pfmsp-3 was amplified by polymerase chain reaction, cloned, and sequenced. Genetic polymorphism and natural selection of Myanmar pfmsp-3 were analysed using the programs DNASTAR, MEGA6, and DnaSP 5.10.00. Genetic diversity and natural selection of the global pfmsp-3 were also comparatively analysed.ResultsMyanmar pfmsp-3 displayed 2 different alleles, 3D7 and K1. The 3D7 allelic type was predominant in the population, but genetic polymorphism was less diverse than for the K1 allelic type. Polymorphic characters in both allelic types were caused by amino acid substitutions, insertions, and deletions. Amino acid substitutions were mainly occurred at the alanine heptad repeat domains, whereas most insertions and deletions were found at the glutamate rich domain. Overall patterns of amino acid polymorphisms detected in Myanmar pfmsp-3 were similar in the global pfmsp-3 population, but novel amino acid changes were observed in Myanmar pfmsp-3 with low frequencies. Complicated patterns of natural selection and recombination events were predicted in the global pfmsp-3, which may act as major driving forces to maintain and generate genetic diversity of the global pfmsp-3 population.ConclusionGlobal pfmsp-3 revealed genetic polymorphisms, suggesting that the functional and structural consequences of the polymorphisms should be considered in designing a vaccine based on PfMSP-3. Further examination of genetic diversity of pfmsp-3 in the global P. falciparum population is necessary to gain in-depth insight for the population structure and evolutionary aspect of global pfmsp-3.

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