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Genetic and molecular characterization of pR351 plasmid from enteropathogenic Escherichia coli strain.

Authors
  • Czarny, A1
  • Lagowska-Złotorzycka, M
  • Mulczyk, M
  • 1 Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Type
Published Article
Journal
Archivum immunologiae et therapiae experimentalis
Publication Date
1989
Volume
37
Issue
1-2
Pages
191–199
Identifiers
PMID: 2695005
Source
Medline
License
Unknown

Abstract

A pR351 plasmid (Tc Ap Cb) conferring drug resistance of naturally occurring E. coli strain was examined. Conjugation and transduction experiments have indicated that this plasmid is R plasmid aggregate consisting of three independent plasmids: a) conjugative plasmid pR351 A (SuTc) fi- (F) belonging to incompatibility group L, b) conjugative plasmid pR351 B (SuApCb) fi- (F) belonging to incompatibility O, and c) non-conjugative plasmid pR351 C (ApCb). The existance of these plasmids in pR351 plasmid aggregate was confirmed by the agarose gel analysis of plasmid DNA isolated from the lysates of E. coli J53 transconjugants carrying pR351 A or pR351 B plasmids and from E. coli K12 C600 transductant carrying pR351 C plasmid. Molecular mass of these plasmids was found to be 55.60 and 3 Mdal respectively. The non-conjugative pR351 C plasmid could be mobilized by Col B and F factors. Our findings have indicated that two fi- (F) R plasmids can stably coexist in R plasmid aggregate.

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