Affordable Access

deepdyve-link
Publisher Website

Genetic loss of AMPK-glycogen binding destabilises AMPK and disrupts metabolism

Authors
  • Hoffman, Nolan J.1
  • Whitfield, Jamie1
  • Janzen, Natalie R.1
  • Belhaj, Mehdi R.1
  • Galic, Sandra2
  • Murray-Segal, Lisa2
  • Smiles, William J.2
  • Ling, Naomi X.Y.2
  • Dite, Toby A.2
  • Scott, John W.1, 2, 3
  • Oakhill, Jonathan S.1, 2
  • Brink, Robert4, 5
  • Kemp, Bruce E.1, 2
  • Hawley, John A.1
  • 1 Exercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia
  • 2 St. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia
  • 3 The Florey Institute of Neuroscience and Mental Health, 30 Royal Parade, Parkville, Victoria 3052, Australia
  • 4 Immunology Division, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, Australia
  • 5 St. Vincent's Clinical School, UNSW Sydney, Level 5 deLacy Building, St. Vincent's Hospital, Darlinghurst, New South Wales 2010, Australia
Type
Published Article
Journal
Molecular Metabolism
Publisher
Elsevier BV
Publication Date
Jun 29, 2020
Volume
41
Identifiers
DOI: 10.1016/j.molmet.2020.101048
PMID: 32610071
PMCID: PMC7393401
Source
PubMed Central
Keywords
License
Unknown

Abstract

• AMPK β subunit knock-in (KI) mice were generated to disrupt glycogen binding in vivo . • Loss of AMPK β2 glycogen binding impairs glucose handling and exercise capacity. • Loss of AMPK β2 glycogen binding increases adiposity. • AMPK β1 and β2 KI mice show increased liver and muscle fat deposition, respectively. • Loss of glycogen binding reduces cellular AMPK protein and kinase activity.

Report this publication

Statistics

Seen <100 times