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Genetic and immunologic characterization of viruses infecting MN-rgp120-vaccinated volunteers.

Authors
  • Phil Berman
  • Am, Gray
  • T, Wrin
  • Jc, Vennari
  • Dj, Eastman
  • Gr, Nakamura
  • Dp, Francis
  • G, Gorse
  • Dh, Schwartz
Type
Published Article
Journal
The Journal of Infectious Diseases
Publisher
Oxford University Press
Volume
176
Issue
2
Pages
384–397
Source
UCSC Bioinformatics biomedical-ucsc
License
Unknown

Abstract

Proviral sequences were determined and immunologic characterization was carried out for envelope glycoproteins from 7 vaccinees who became infected with human immunodeficiency virus type 1 (HIV-1), through high-risk behavior, while participating in clinical trials of MN-rgp120, a candidate HIV-1 vaccine. All 7 infections resulted from subtype B viruses; however, only 3 of the viruses possessed the MN serotype-defining V3 domain sequence, IGPGRAF, prevalent in 60%-70% of US infections. Six of the 7 viruses differed from MN-rgp120 at a neutralizing epitope in the C4 domain, and all 7 differed from MN-rgp120 at a neutralizing epitope in the V2 domain. Recombinant gp120 was prepared from each breakthrough specimen and tested for binding to a panel of neutralizing monoclonal antibodies. The results suggest that 6 of 7 breakthrough infections may be related to incomplete immunization or to infection with viruses that differed from the vaccine immunogen at important virus-neutralizing epitopes.

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