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Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China.

Authors
  • He, Li1, 2
  • Fang, Zheng-Ming3
  • Xue, Ting1
  • Zhang, Er-Fu1
  • An, Chun-Li1
  • 1 Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University, Shenyang 110122, PR. China. , (China)
  • 2 Department of Inspection, 4th Affiliated Hospital of China Medical University, Shenyang 110032, PR. China. , (China)
  • 3 Department of Parasitology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR. China. , (China)
Type
Published Article
Journal
The Korean journal of parasitology
Publication Date
Jun 01, 2019
Volume
57
Issue
3
Pages
309–312
Identifiers
DOI: 10.3347/kjp.2019.57.3.309
PMID: 31284356
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97-100% similarity to the reference sequence of S. erinaceieuropaei and 88-89% to the reference sequence of S. decipiens. There were 1-12 bases different between these worms, but no obvious genetic variation (0-3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1-3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.

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