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Genetic dissection of climacteric fruit ripening in a melon population segregating for ripening behavior

Authors
  • Pereira, Lara1
  • Santo Domingo, Miguel1
  • Ruggieri, Valentino1, 2
  • Argyris, Jason1, 2
  • Phillips, Michael A.3
  • Zhao, Guangwei4
  • Lian, Qun5
  • Xu, Yongyang4
  • He, Yuhua4
  • Huang, Sanwen5
  • Pujol, Marta1, 2
  • Garcia-Mas, Jordi1, 2
  • 1 Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Edifici CRAG, Campus UAB, Cerdanyola, Barcelona, 08193, Spain , Cerdanyola (Spain)
  • 2 IRTA (Institut de Recerca i Tecnologia Agroalimentàries), Edifici CRAG, Campus UAB, 08193 Cerdanyola, Barcelona, Spain , 08193 Cerdanyola (Spain)
  • 3 University of Toronto–Mississauga, Mississauga, ON, L5L 1C6, Canada , Mississauga (Canada)
  • 4 Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China , Zhengzhou (China)
  • 5 Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China , Shenzhen (China)
Type
Published Article
Journal
Horticulture Research
Publisher
Nature Publishing Group UK
Publication Date
Nov 01, 2020
Volume
7
Issue
1
Identifiers
DOI: 10.1038/s41438-020-00411-z
Source
Springer Nature
License
Green

Abstract

Melon is as an alternative model to understand fruit ripening due to the coexistence of climacteric and non-climacteric varieties within the same species, allowing the study of the processes that regulate this complex trait with genetic approaches. We phenotyped a population of recombinant inbred lines (RILs), obtained by crossing a climacteric (Védrantais, cantalupensis type) and a non-climcteric variety (Piel de Sapo T111, inodorus type), for traits related to climacteric maturation and ethylene production. Individuals in the RIL population exhibited various combinations of phenotypes that differed in the amount of ethylene produced, the early onset of ethylene production, and other phenotypes associated with ripening. We characterized a major QTL on chromosome 8, ETHQV8.1, which is sufficient to activate climacteric ripening, and other minor QTLs that may modulate the climacteric response. The ETHQV8.1 allele was validated by using two reciprocal introgression line populations generated by crossing Védrantais and Piel de Sapo and analyzing the ETHQV8.1 region in each of the genetic backgrounds. A Genome-wide association study (GWAS) using 211 accessions of the ssp. melo further identified two regions on chromosome 8 associated with the production of aromas, one of these regions overlapping with the 154.1 kb interval containing ETHQV8.1. The ETHQV8.1 region contains several candidate genes that may be related to fruit ripening. This work sheds light into the regulation mechanisms of a complex trait such as fruit ripening.

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