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Genetic Characterization of Clostridium botulinum Isolated from the First Case of Infant Botulism in Korea

Authors
  • Jeon, Jun Ho
  • Choi, Chi-Hwan
  • Kim, Jeong Hyun
  • Hyun, Junghee
  • Choi, Eun-Sun
  • Choi, Sang-Yoon
  • Shin, Yong-Woo
  • Pyo, Seong Wook
  • Kim, Dae-Won
  • Kang, Byung Hak
  • Park, Young Joon
  • Rhie, Gi-eun
Type
Published Article
Journal
Annals of Laboratory Medicine
Publisher
Korean Society for Laboratory Medicine
Publication Date
Sep 01, 2021
Volume
41
Issue
5
Pages
489–492
Identifiers
DOI: 10.3343/alm.2021.41.5.489
PMID: 33824238
PMCID: PMC8041589
Source
PubMed Central
Keywords
Disciplines
  • Brief Communications
  • Clinical Chemistry
License
Unknown

Abstract

Botulism is a neuroparalytic disease caused by a neurotoxin produced by Clostridium botulinum . This study aimed to genetically characterize C. botulinum strain isolated from the first case of infant botulism in Korea reported on June 17, 2019. We isolated C. botulinum strain CB-27 from a stool sample of the patient and analyzed the toxin types and toxin gene cluster compositions of the strain using a mouse bioassay, real-time PCR, and genome sequencing. Toxin gene cluster analysis showed that strain CB-27 possesses a C. botulinum neurotoxin type A harboring an unexpressed B gene. Although the nucleotide and amino acid sequences of toxin genes as well as the toxin gene cluster arrangements in strain CB-27 were identical to those of the known strain CDC_69094, the total nucleotide sequences of the toxin gene clusters of CB-27 differed from those of CDC_69094 by 0.47%, indicating genetic diversity of toxin gene clusters of CB-27 among other previously reported C. botulinum strains. To our knowledge, this is the first description of a C. botulinum strain with two separate toxin gene clusters in Korea.

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