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Generic model control of the specific growth rate in recombinant Escherichia coli cultivations.

Authors
Type
Published Article
Journal
Journal of Biotechnology
0168-1656
Publisher
Elsevier
Publication Date
Volume
122
Issue
4
Pages
483–493
Identifiers
PMID: 16293333
Source
Medline
License
Unknown

Abstract

Generic model control is shown to be a powerful tool for keeping a microbial cultivation process close to its predetermined (optimized) control profile. This is demonstrated at the example of the green fluorescent protein expressed in genetically modified Escherichia coli host cells. It is shown that the process can be run very closely to a predefined complex profile of the specific cell growth rate mu(t). Controlling the experiments at many different growth conditions is a straightforward way of effectively collecting the data necessary for optimization of recombinant protein production systems. Although the process dynamics is rather complex, the model for the controller can be kept quite simple. The control technique, used here for specific growth rate control, is quite universal and can be applied for different biotechnological processes as well.

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