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Generation-dependent effect of PAMAM dendrimers on human insulin fibrillation and thermal stability.

Authors
  • Nowacka, Olga1
  • Milowska, Katarzyna2
  • Belica-Pacha, Sylwia3
  • Palecz, Bartlomiej3
  • Šipošová, Katarina4
  • Gazova, Zuzana4
  • Bryszewska, Maria1
  • 1 Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland. , (Poland)
  • 2 Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland. Electronic address: [email protected] , (Poland)
  • 3 Department of Physical Chemistry, University of Lodz, 165 Pomorska St., 90-236, Poland. , (Poland)
  • 4 Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, 47 Watsonova St., 040 01 Kosice, Slovakia. , (Slovakia)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Jan 01, 2016
Volume
82
Pages
54–60
Identifiers
DOI: 10.1016/j.ijbiomac.2015.10.029
PMID: 26598047
Source
Medline
Keywords
License
Unknown

Abstract

We have studied the effect of polyamidoamine (PAMAM) dendrimers of various generations on the thermal stability and fibrillation of human insulin. Thermostability of human insulin used differential scanning calorimetry (DSC), which showed two phase-transitions for insulin at 60 and 82°C. After adding dendrimers at 0.6 μmol/l, the first peaks disappeared and the second peaks were higher. We posited that, in the presence of dendrimers, the dimers in the solution were transformed into hexamers. The effect of dendrimers on insulin fibrillation was monitored by measuring ThT fluorescence, and visualization of insulin fibrils by transmission electron microscopy (TEM) and atomic force microscopy (AFM). The effect of PAMAM dendrimers on insulin fibrillation was strongly dependent on the dendrimers generation and dendrimer:protein ratio.

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