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Generation of viable progeny from dead brooders of endangered catfish Clarias magur (Hamilton, 1822).

Authors
  • Majhi, Sullip Kumar1
  • Kumar, Santosh1
  • 1 ICAR-National Bureau of Fish Genetic Resources, Division of Fish Conservation, Canal Ring Road, Dilkhusa P.O., Lucknow, Uttar Pradesh 226002, India. , (India)
Type
Published Article
Journal
Heliyon
Publisher
Elsevier
Publication Date
Oct 01, 2017
Volume
3
Issue
10
Identifiers
DOI: 10.1016/j.heliyon.2017.e00434
PMID: 29226264
Source
Medline
Keywords
License
Unknown

Abstract

The obligatory air-breathing catfish Clarias magur is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature C. magur brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature C. magur. Three hours after death, the gonads were excised, macroscopically examined and gamete viability was evaluated. Artificial fertilization was performed by mixing the sperm suspension with the eggs. Water was added after 1 min of mixing to activate the fertilization process. We observed 85%-93% fertilization success from gametes derived from dead donors as opposed to 90%-95% from those derived from live control donors. The embryos showed normal development and resulted in the generation of 88%-92% viable progeny, which was similar to the progeny derived from control donors (92%-93%). The results obtained in this study will have profound implications in enhancing the seed production of endangered C. magur and could potentially be applied to other key commercially or endangered fish species.

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