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Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.

Authors
  • M Yamamoto
  • J M Jones
  • E Senghas
  • C Gawron-Burke
  • D B Clewell
Publication Date
May 01, 1987
Source
PMC
Keywords
License
Unknown

Abstract

A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA containing the 16-kilobase conjugative transposon Tn916. It was possible to introduce some of the resulting Tn916::Tn5 derivatives back into S. faecalis by using a recently developed protoplast transformation procedure. A presumed zygotic induction resulted in insertion of the Tn916 derivatives at multiple sites in the S. faecalis chromosome.

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