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Generation of deletion lines in allohexaploid Bread Wheat

Authors
  • Serra, Heïdi
  • Svačina, Radim
  • Bartoš, Jan
  • Sourdille, Pierre
Publication Date
Apr 24, 2022
Identifiers
DOI: 10.1007/978-1-0716-2253-7_14
OAI: oai:HAL:hal-03656818v1
Source
HAL
Keywords
Language
English
License
Unknown
External links

Abstract

Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopolyploidy. One way to face this challenge is to decrease the size of the interval bearing the gene of interest both genetically and physically, in order to reduce significantly the number of potential candidate genes. In this chapter, we describe a technical approach to produce chromosome-specific deletion lines to locate precisely genes of interest onto wheat chromosomes, a step forward to their cloning.

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