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Generation of blood-derived dendritic cells in dogs with oral malignant melanoma.

Authors
  • Catchpole, B
  • Stell, A J
  • Dobson, J M
Type
Published Article
Journal
Journal of comparative pathology
Publication Date
Jan 01, 2002
Volume
126
Issue
2-3
Pages
238–241
Identifiers
PMID: 11945016
Source
Medline
License
Unknown

Abstract

Advances in treatment of human melanoma indicate that immunotherapy, particularly dendritic cell (DC) immunization, may prove useful. The aim of this study was to investigate whether blood-derived DCs could be generated from canine melanoma patients. Peripheral blood mononuclear cells were isolated from three such dogs and cultured with recombinant canine granulocyte-macrophage colony stimulating factor (GM-CSF), canine interleukin 4 and human Flt3-ligand for 7 days. The resulting cells demonstrated a typical dendritic morphology, and were enriched for cells expressing CD1a, CD11c and MHC II by flow cytometric analysis. Thus, canine blood-derived DCs can be generated in vitro and DC immunization should be feasible in dogs.

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