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Generating nonlinear concentration gradients in microfluidic devices for cell studies.

Authors
  • Selimović, Šeila
  • Sim, Woo Young
  • Kim, Sang Bok
  • Jang, Yun Ho
  • Lee, Won Gu
  • Khabiry, Masoud
  • Bae, Hojae
  • Jambovane, Sachin
  • Hong, Jong Wook
  • Khademhosseini, Ali
Type
Published Article
Journal
Analytical Chemistry
Publisher
American Chemical Society
Publication Date
Mar 15, 2011
Volume
83
Issue
6
Pages
2020–2028
Identifiers
DOI: 10.1021/ac2001737
PMID: 21344866
Source
Medline
License
Unknown

Abstract

We describe a microfluidic device for generating nonlinear (exponential and sigmoidal) concentration gradients, coupled with a microwell array for cell storage and analysis. The device has two inputs for coflowing multiple aqueous solutions, a main coflow channel and an asymmetrical grid of fluidic channels that allows the two solutions to combine at intersection points without fully mixing. Due to this asymmetry and diffusion of the two species in the coflow channel, varying amounts of the two solutions enter each fluidic path. This induces exponential and sigmoidal concentration gradients at low and high flow rates, respectively, making the microfluidic device versatile. A key feature of this design is that it is space-saving, as it does not require multiplexing or a separate array of mixing channels. Furthermore, the gradient structure can be utilized in concert with cell experiments, to expose cells captured in microwells to various concentrations of soluble factors. We demonstrate the utility of this design to assess the viability of fibroblast cells in response to a range of hydrogen peroxide (H(2)O(2)) concentrations.

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