General transduction in Rhizobium meliloti by a thermosensitive mutant of bacteriophage DF2.
- Authors
- Publication Date
- Jul 01, 1979
- Source
- PMC
- Keywords
- License
- Unknown
Abstract
A simiplified version of bacteriophage DF2 transduction, using the thermosensitive mutant DF2tsC, is described. The transduction frequencies of Rhizobium meliloti markers by DF1tsC are of about 10(-6) or higher. The development of transductants at 34 degrees C does not require the addition of antiserum for phage neutralization.