Affordable Access

deepdyve-link deepdyve-link
Publisher Website

General introduction: recombinant protein production and purification of insoluble proteins.

Authors
  • Ferrer-Miralles, Neus
  • Saccardo, Paolo
  • Corchero, José Luis
  • Xu, Zhikun
  • García-Fruitós, Elena
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2015
Volume
1258
Pages
1–24
Identifiers
DOI: 10.1007/978-1-4939-2205-5_1
PMID: 25447856
Source
Medline
License
Unknown

Abstract

Proteins are synthesized in heterologous systems because of the impossibility to obtain satisfactory yields from natural sources. The production of soluble and functional recombinant proteins is among the main goals in the biotechnological field. In this context, it is important to point out that under stress conditions, protein folding machinery is saturated and this promotes protein misfolding and, consequently, protein aggregation. Thus, the selection of the optimal expression organism and the most appropriate growth conditions to minimize the formation of insoluble proteins should be done according to the protein characteristics and downstream requirements. Escherichia coli is the most popular recombinant protein expression system despite the great development achieved so far by eukaryotic expression systems. Besides, other prokaryotic expression systems, such as lactic acid bacteria and psychrophilic bacteria, are gaining interest in this field. However, it is worth mentioning that prokaryotic expression system poses, in many cases, severe restrictions for a successful heterologous protein production. Thus, eukaryotic systems such as mammalian cells, insect cells, yeast, filamentous fungus, and microalgae are an interesting alternative for the production of these difficult-to-express proteins.

Report this publication

Statistics

Seen <100 times