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A general approach for the generation of orthogonal tRNAs.

Authors
  • Wang, L
  • Schultz, P G
Type
Published Article
Journal
Chemistry & Biology
Publisher
Elsevier
Publication Date
Sep 01, 2001
Volume
8
Issue
9
Pages
883–890
Identifiers
PMID: 11564556
Source
Medline
License
Unknown

Abstract

The mutant suppressor tRNA(Tyr)(CUA) together with the M. jannaschii TyrRS is an excellent orthogonal tRNA-synthetase pair for the in vivo incorporation of unnatural amino acids into proteins. This general approach may be expanded to generate additional orthogonal tRNA-synthetase pairs as well as probe the interactions between tRNAs and their cognate synthetases.

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