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Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease.

Authors
  • Mourkas, Evangelos1
  • Florez-Cuadrado, Diego2
  • Pascoe, Ben1, 3
  • Calland, Jessica K1
  • Bayliss, Sion C1, 3
  • Mageiros, Leonardos1
  • Méric, Guillaume1, 4, 5
  • Hitchings, Matthew D6
  • Quesada, Alberto7
  • Porrero, Concepción2
  • Ugarte-Ruiz, María2
  • Gutiérrez-Fernández, José8
  • Domínguez, Lucas2, 9
  • Sheppard, Samuel K1, 3, 10
  • 1 The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, BA27AY, Bath, UK.
  • 2 VISAVET Health Surveillance Centre, Universidad Complutense Madrid, Madrid, Spain. , (Spain)
  • 3 MRC CLIMB Consortium, University of Bath, Bath, UK.
  • 4 Cambridge Baker Systems Genomics Initiative, Baker Heart and Diabetes Institute, 75 Commercial Rd, Melbourne, 3004, Victoria, Australia. , (Australia)
  • 5 Department of Infectious Diseases, Central Clinical School, Monash University, Melbourne, Victoria, 3004, Australia. , (Australia)
  • 6 Swansea University Medical School, Swansea, Swansea, UK.
  • 7 Department of Biochemistry, Molecular Biology and Genetics, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain. , (Spain)
  • 8 Department of Microbiology, University of Granada, Granada, Spain. , (Spain)
  • 9 Department of Animal Health, Faculty of Veterinary Medicine, Universidad Complutense Madrid, Madrid, Spain. , (Spain)
  • 10 Department of Zoology, University of Oxford, Oxford, UK.
Type
Published Article
Journal
Environmental Microbiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Dec 01, 2019
Volume
21
Issue
12
Pages
4597–4613
Identifiers
DOI: 10.1111/1462-2920.14760
PMID: 31385413
Source
Medline
Language
English
License
Unknown

Abstract

The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here, we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. AMR was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug-resistant genomic islands (GIs), often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment. © 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

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