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Gene Expression Profiles Induced by High-dose Ionizing Radiation in MDA-MB-231 Triple-negative Breast Cancer Cell Line.

Authors
  • Bravatà, Valentina1
  • Cammarata, Francesco Paolo1
  • Minafra, Luigi2
  • Musso, Rosa1
  • Pucci, Gaia1
  • Spada, Massimiliano3
  • Fazio, Ivan4
  • Russo, Giorgio1
  • Forte, Giusi Irma1
  • 1 Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle Ricerche (IBFM-CNR), Cefalù, Italy. , (Italy)
  • 2 Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle Ricerche (IBFM-CNR), Cefalù, Italy [email protected] , (Italy)
  • 3 Oncology Unit, Fondazione Istituto G. Giglio, Cefalù, Italy. , (Italy)
  • 4 Casa di Cura Macchiarella, Palermo, Italy. , (Italy)
Type
Published Article
Journal
Cancer Genomics & Proteomics
Publisher
International Institute of Anticancer Research
Publication Date
Jan 01, 2019
Volume
16
Issue
4
Pages
257–266
Identifiers
DOI: 10.21873/cgp.20130
PMID: 31243106
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Radiation therapy (RT) represents a therapeutic option in breast cancer (BC). Even if a great number of BC patients receive RT, not all of them report benefits, due to radioresistance that gets activated through several factors, such as the hormone receptor status. Herein, we analyzed the gene expression profiles (GEP) induced by RT in triple-negative BC (TNBC) MDA-MB-231, to study signalling networks involved in radioresistance. GEP of MDA-MB-231 BC cells treated with a high dose of radiation, went through cDNA microarray analysis. In addition, to examine the cellular effects induced by RT, analyses of morphology and clonogenic evaluation were also conducted. A descriptive report of GEP and pathways induced by IR is reported from our microarray data. Moreover, the MDA-MB-231 Radioresistent Cell Fraction (RCF) selected, included specific molecules able to drive radioresistance. In summary, our data highlight, the RT response of TNBC MDA-MB-231 cell line at a transcriptional level, in terms of activating radioresistance in these cells, as a model of late-stage BC. Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

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